Journal: Molecular Therapy Advances

Article Title: Systems vaccinology analysis of saRNA immunization identifies an acute innate immune signature correlated with adaptive immunity

doi: 10.1016/j.omta.2026.201706

Figure Lengend Snippet: Adaptive immune responses to vaccination correlate weakly with innate responses 24 h after prime immunization Adaptive immune response data are presented from all participants pooled (A–C) or from participants separated into those who received 1 or 5 μg doses (D–F). (A and D) Anti-Spike (S) IgG (ng/mL) in sera from participants receiving two doses of LNP-saRNA at various time points after enrollment. (B and E) Pseudoneutralizing antibody IC50 from participants receiving two doses of LNP-saRNA; responses are shown at 6 weeks after enrollment against Wuhan, Delta, and Omicron spike-expressing pseudoviruses. (C and F) IFN-γ spot forming units (SFU) per million cells (ELISpot) from PBMC stimulated with SARS-CoV-2 spike peptide pools. (G) Correlation between V2a chemokine levels and the last visit antibody response. (H) Correlation between V2a cell levels and the last visit antibody response. Points represent individual participants, bars represent median ± interquartile range (A–F); points represent individual participants.

Article Snippet: Pre-coated IFN- γ ELISpot 96-well plates (Mabtech) were washed with sterile PBS and blocked with R10 media before the addition of 50 μL of cells per well in triplicate, with 50 μL media only, stimulation media containing vaccine-specific peptide pools Env 1, 2, and 3 (15-mers overlapping by 11, covering the entire sequence of SARS-CoV-2 spike matched to the vaccine insert), and two positive controls, PHA (Sigma-Aldrich) and CEFX Ultra SuperStim Pool (JPT, Berlin, Germany), at a final concentration of 2.5 μg/mL.

Techniques: Expressing, Enzyme-linked Immunospot